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Subcellular localisation of a Hig2-EGFP fusion protein and mutational analysis of the putative signal peptide in HC11 and MDCK cells. (a and c) Hig2-EGFP is localised to large spherical vesicles located in the cytoplasm. (b and d) The introduction of two aspartate residues into the putative hydrophobic signal peptide abolishes the vesicular subcellular localisation of the mutant protein.
