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. 2017 Jun 1;20(6):771–784.e6. doi: 10.1016/j.stem.2017.02.009

Figure 4.

Figure 4

Distinct Roles of Progenitor Populations during Hematopoietic Recovery

(A) Schematic depiction of competitive hematopoietic repopulation assay.

(B and C) H&E stains of injected tibiae (B), and tdTomato (red) and Perilipin (green) (C) IF co-localization on adipocytes in injected tibiae (green arrow, host derived; orange arrow, donor derived). Scale bars, 50 μm.

(D) Fate analyses of transplanted cells. CD45−CD31−tdTomato+ cells (red squares, top row) were gated for expression of GFP (green squares, Zfp423-EGFP reporter; middle row). Zfp423+ cells were then assessed for expression of surface markers Sca1 and CD24 (bottom row).

(E–G) Bone marrow cellularity (E), marrow chimerism (F), and donor-derived (G) LSK cells in injected and contralateral tibiae 5 weeks after irradiation.

(H and I) Donor-derived LT-LSK (H) and donor-derived ST-LSK (I) hematopoietic stem cells in injected tibiae. Mean ± SEM (n = 7); p < 0.05: a, versus no cells; b, versus OPC; c, versus CD31−CD45−Sca1+CD24+; d, versus APC; and e, versus preAd.

See also Figure S5.