Figure 5.

The Adipocytic Lineage Inhibits Bone Healing

(A) Red fluorescence in tibiae (top panels), μCT images (middle panels), and representative Movat-Pentachrome stains (lower panels; yellow, mineralized bone; red, new bone; blue, cartilage; purple, nuclei) of fracture calluses 14 days after fracture and intratibial injection of the indicated cell populations.

(B) Total bone mineral density (BMD) by μCT (n = 6).

(C–E) Histomorphometry of mineralized bone volume (BV) and non-mineralized callus (C) as fibrous tissue (FV) (D), and cartilage tissue (CV) volumes (E), all normalized to total callus volume (TV) (n = 8). Mean ± SEM; p < 0.05: a, versus no cells; b, versus OPC; and c, versus CD31⁻CD45⁻Sca1⁺CD24⁺. Scale bar, 100 μm.

See also Figure S6.