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. 2017 Jun 5;7:2782. doi: 10.1038/s41598-017-03094-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Peritoneal fluid humoral and cellular analysis. (A) Detection of F. hepatica cathepsin L1 (FhCL1) specific antibodies in peritoneal fluid by immunoblotting. Lane 1: FhCL1 positive control; Lane 2: peritoneal fluid from the uninfected pool (UI_PF); Lane 3: peritoneal fluid from the infected pool (I_PF). (B) IgG level response in peritoneal fluid against F. hepatica cathepsin L1 (FhCL1). 1: FhCL1 positive control; 2: uninfected (UI_PF); 3: infected (I_PF). (C) Total mean cell count per ml in the peritoneal fluid from the uninfected (UI) and infected (I) groups (n = 5; ± standard deviation is represented). (D) Mean differential cell count showing the percentages of macrophages, lymphocytes, neutrophils and eosinophils in the peritoneal fluid of uninfected and infected sheep (n = 5; ± standard deviation is represented). (E) Mean differential cell count of macrophages, lymphocytes, neutrophils and eosinophils in the peritoneal fluid of uninfected and infected sheep (n = 5; ± standard deviation) represented by cell number. *P value < 0.05; **P value < 0.01.