Table 1.

Primers for quantitative PCR (qPCR) cytokine analysis.

Cytokine	Forward sequence 5′-3′	Reverse sequence 5′-3′	Reference
IFN-γ	ATCTCTTTCGAGGCCGGAGA	ATTGCAGGCAGGAGAACCAT	110
IL-1	CACTGCCAGAAAATAAGCTGAAAC	TGATCAAGCAAATCGCCTGAT	111
IL-4	GGAGCTGCCTGTAGCAGACG	TTCTCAGTTGCGTTCTTTGGG	110
IL-5	CTGCTGATAGGTGATGGGAACTT	GGTGATTTGTATGCTGAGGAGTAGG	110
IL-10	CTGAGAACCATGGGCCTGAC	TCTCCCCCAGCGAGTTCAC	110
IL-12	GAATTCTCGGCAGGTGGAAG	GTGCTCCACGTGTCAGGGTA	110
IL-13	AGAACCAGAAGGTGCCGCT	GGTTGAGGCTCCACACCATG	110
IL-17	TGTGAGGGTCAACCTGAACAT	TGATAATCGGTGGGCCTTCTG	111
IL-23	GGGAAGTGGACAGAGGTTCC	CTGCCTCTCCAATCTGGGTG	112
TNF-α	CCCGTCTGGACTTGGATCCT	TGCTTTTGGTGCTCATGGTG	110
TGFβ1	GAACTGCTGTGTTCGTCAGC	GGTTGTGCTGGTTGTACAGG	113
Arginase	GCGGAAGTCAAGAAGACTGG	AGGTTGTCCATGCAAGTTCC	Current study±
iNOS	TAGAGGAACATCTGGCCAGG	TGGCAGGGTCCCCTCTGATG	Current study^
Eotaxin	ACAAGAAAATCTGTGTTGATCCCC	CCATGGCATTCTGGACCC	111
B2M	TTCTGTCCCACGCTGAGTTCA	CAACCCAAATGAGGCATCGT	*
Β-actin	ACCAGTTCGCCATGGATGA	AGCCGTTGTCAACCACGAG	110
GAPDH	GGTGATGCTGGTGCTGAGTA	TCATAAGTCCCTCCACGATG	111

*Pacheco, I.L., personal communication. ^±Based on Ovis aries arginase 1 (XM_004011324). ^Based on the bovine sequences from Adler et al.¹¹⁴ using the Ovis aries nitric oxide synthase 2 gene (XM_004012488).