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. 2017 Jun 5;7:2800. doi: 10.1038/s41598-017-03012-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression patterns of mam and mms genes under high- and low-iron conditions. (A,B,C) Fold changes of expression of mamAB operon, mamXY operon, mamC, and mms6 at 18 h. No clear expression differences for 12 MAI genes were observed under the two conditions at 18 h. (D) Representation of “expression rules” from the Multi Experiment Viewer program, V. 4.8.1. The expression matrix is a false-color view on a red-green scale (green = low expression; red = high expression). (E) K-means clustering shows a consistent trend of expression for members of each cluster. Twelve MAI genes increased at the earlier log phase (6–12 h) of magnetosome formation. The products (proteins) of these genes are located on magnetosome membrane, and may be involved in cell growth and magnetosome formation at the lag phase (0–6 h).