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. 2017 Jun 5;7:2807. doi: 10.1038/s41598-017-02901-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NKX6.3 reduces ROS levels by regulating ROS-responsive genes. (A–C) Measurement of ROS using Fluorescence microscopy and FACS analysis by DCFDA staining. Stable expression of NKX6.3 in AGS and MKN1 cells showed reduced levels of ROS in a time-dependent manner, compared to mock stable AGS and MKN1 cells. Results are represented as mean ± SEM from three independent experiments. (D) NKX6.3 significantly induced the expression of Hace1, Nrf2, catalase and mnSOD, and reduced the expression of Nox1 and Noxa1 proteins in western blot assay. (E) In real-time PCR analysis, NKX6.3 induced GSH, Nqo-1 and Ho-1 mRNA transcript. Results are represented as mean ± SEM from three independent experiments. Each dot plot represents the result from the individual experiment. ***P < 0.0001, *P < 0.05, based on the student’s t-test.