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. 2017 Jun 5;7:2787. doi: 10.1038/s41598-017-02632-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The number of informative reads at different lengths for sample SU3. (b) The number of informative reads at different lengths for sample SU3 that were mapped to known rat miRNAs. (c) Principal component analysis of 11 samples based on RPM expression of 219 differentially expressed miRbase miRNAs. (d and e) Heatmaps of 56 upregulated miRNAs in PSCs (d) and 59 upregulated miRNAs in EFs (e). Known miRNAs with an extremum fold change more than 10, and the log2 transformation.