Table 2.
Novel miRNA differentially expressed between PSCs and EFs.
| Position (rn5) | Shoulder | PSC or EF | iPSC, RPM | ESC, RPM | EF, RPM | Homolog | miRdeep2 | Custom | Pre-miRNA primary sequence |
|---|---|---|---|---|---|---|---|---|---|
| chr5:+:152026238–152026277 | 3p | EF > PSC | 0 | 0 | 2.98 | 1 | 0 | AAGGGGCAGGTTGCTGATACATTGTGACATAGACTTGGAAACAGTTGGTAATAGTGTACTGTGACCTGTCCTAGGGTGA | |
| chr8:+:67350015–67350048 | 3p | EF > PSC | 0 | 0 | 6.31 | 1 | 0 | CTCAGACATGGTTCCTGTCCTCTTGGGTCTCAGTATTGATGTGGAGTACAGAGCACATGTTTGAGGGG | |
| chr8:+:67395497–67395530 | 3p | EF > PSC | 0 | 0 | 6.31 | 1 | 1 | TCAGACATGGTTCCTGTCCTCTTGGGTCTCAGTATTGATGTGGAGTACAGAGCACATGTTTGAGGGG | |
| chr5:+:83208017–83208048 | 5p | EF > PSC | 0 | 0 | 3.65 | 1 | 1 | TGGTCATGGTTCATGAACTGGAGCGGGTCCATCGTGTGTGTTCCAGTTCAACCTTGTCCCTGTT | |
| chr3:−:124104501–124104531 | 5p | EF > PSC | 0 | 0 | 3.26 | 1 | 0 | CATGTTCCACTCACTCTCAGACGAAAACAGAGAGGCTCTGGGAGAGGTGTGGGACCAGTAC | |
| chr10:−:32125896–32125927 | 5p | EF > PSC | 0 | 0 | 2.64 | 1 | 0 | TCTGTGAGTCTGAAGCCCACCTGGTTTACATATCAAGTTCCAAGTGCGCCAGACCCACATAGTG | |
| chr6:−:137837930−137837960 | 5p | EF > PSC | 2.34 | 0.63 | 15.73 | 1 | 0 | TCTGCTCCTGCTCTTTCTGCTGCTGCTGTTCCTGTTGCCAGCGGGCGAGGAGGAGCGCAGCT | |
| chr2:+:217974496–217974523 | 5p | PSC > EF | 17.74 | 17.01 | 2.66 | 1 | 0 | ATTGCCTGGTTTAGTCTCTGCTACTTGCAAGTACCAGGTCACTAAAACAGGCAGGG | |
| chrX:−:151288045–151288072 | 3p | PSC > EF | 11656.24 | 11023.61 | 3.90 | 1 | 0 | TACTCAGATTAGTGTCACTCCAGGACATAAATATGTATGGCGCTCCTCTGAGTAGAA | |
| chr14:+:26226537–26226565 | 5p | PSC > EF | 61.89 | 72.76 | 0 | 1 | 1 | TGCTTGCCTGGATTACAGTGAAGGTATTCATGTTTCACTGCACTCTAGACAGGCATTA | |
| chrX:−:151288073–151288101 | 5p | PSC > EF | 107.52 | 169.08 | 0 | 1 | 0 | TACTCAGATTAGTGTCACTCCAGGACATAAATATGTATGGCGCTCCTCTGAGTAGAA | |
| chr1:−:63523357–63523389 | 3p | PSC > EF | 3395.86 | 3010.64 | 0.97 | MIR372 | 1 | 1 | CACTCAAATGTAGGAGCGCTCTTCTGATCTGGTTTAAGTGGAAAGTGTTGCATCGTTTGGGTGTCAT |
| chr1:−:63523390–63523423 | 5p | PSC > EF | 243.23 | 199.24 | 0.33 | MIR372 | 1 | 1 | CACTCAAATGTAGGAGCGCTCTTCTGATCTGGTTTAAGTGGAAAGTGTTGCATCGTTTGGGTGTCAT |
| chr2:+:251220594–251220625 | 3p | PSC > EF | 45.96 | 47.38 | 0 | MIR302B | 1 | 0 | CCACTTTAACATGGGAATGCTTTCTGTCACATTGAAGAGTAAGTGCTGCCATGTTTTAGTAGA |
| chr2:+:251220844–251220873 | 3p | PSC > EF | 37.22 | 30.67 | 0 | MIR302 | 1 | 1 | TAAACGTGGTTGTCCTTGCTTTGGAACGAAGAAAGTAAGTGCTTCCATGTTTGGGTGAT |
| chr1:+:148620388–148620416 | 5p | PSC > EF | 95.17 | 32.11 | 0.97 | MIR147B | 1 | 0 | GTGGAAACACTTCTGCACAAACTCGATTTTGATGCCAGTGTGCAGAAATGCTTCTGCT |
| chrX:−:140167614–140167645 | 5p | PSC > EF | 18.99 | 31.98 | 0.67 | MIR18B | 0 | 1 | TAAGGTGCATCTAGTGCTGTTAGTGAAGCAGCTTATAATCTACTGCCCTAAATGCCCCTTCTCG |
| chr7:+:70463555–70463594 | 5p | EF > PSC | 2640.99 | 2751.3 | 9939.24 | MIR26 | 0 | 1 | GAGAGCCGGCTGTGGCTGGATTCAAGTAATCCAGGATAGGCTGTTTCCATCTGTGAGGCCTATTCTTGATTACTTGTTTCT |
| chr7:+:70463595–70463635 | 3p | EF > PSC | 2609.2 | 2734.29 | 9854.73 | MIR26 | 0 | 1 | GAGAGCCGGCTGTGGCTGGATTCAAGTAATCCAGGATAGGCTGTTTCCATCTGTGAGGCCTATTCTTGATTACTTGTTTCT |
| chr3:+:16697079–16697110 | 5p | EF > PSC | 8.53 | 7.95 | 2241.76 | MIR199 | 0 | 1 | CCCAGTGTTTAGACTACCTGTTCAGGACTCCCAAATTGTACAGTAGTCTGCACATTGGTTAGGCT |
| chr3:+:16697111–16697143 | 3p | EF > PSC | 81.95 | 73.78 | 14456.4 | MIR199 | 0 | 1 | CCCAGTGTTTAGACTACCTGTTCAGGACTCCCAAATTGTACAGTAGTCTGCACATTGGTTAGGCT |
| chrX:+:105464335–105464360 | 5p | EF > PSC | 0 | 0 | 3.3 | Mir7093 | 1 | 0 | TTTCCATCTGTCACCCTGCAGGTCTGCTGTAGCAGGTGGCACTGCTTGAAGCA |
| chr14:−:30139894–30139924 | 3p | EF > PSC | 1.74 | 0 | 74.28 | MIR365 | 0 | 1 | GGGACTTTTGGGGGCAGATGTGTTTCCATTCCACTATCATAATGCCCCTAAAAATCCTTATTG |