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. 2017 Jun 5;7:2798. doi: 10.1038/s41598-017-02935-2

Figure 5.

Figure 5

Downregulation of UHRF1 activates CXCR4/IL-6/JAK/STAT3 pathway. (a) Western blot analysis for the phosphorylation status of STAT3 in shCont- and shUHRF1-HepG2 cells. (b and c) Effects of AG490 on the migratory and invasive properties of shUHRF1-HepG2 cells. The cells were grown in the presence or absence of AG490 (5 μM) for 48 h, and the migration and invasion assay were performed using the Transwell chamber. (d) Effects of AG490 on the protein expression level of Snail and vimentin in shUHRF1-HepG2 cells. (e) Effects of siRNA targeting CXCR4 on the protein expression level of phosphorylated STAT3 in shUHRF1-HepG2 cells. The cells were grown in the presence or absence of siRNA targeting CXCR4 for 48 h, and western blot analysis was performed. (f) Effects of siRNA targeting IL-6 on the protein expression level of phosphorylated STAT3 in shUHRF1-HepG2 cells. The cells were grown in the presence or absence of siRNA targeting IL-6 for 48 h, and western blot analysis was performed. β-actin was used as a loading control. Results from three independent experiments are expressed as means ± SEMs. (*P < 0.05, **P < 0.01).