Table 2.
Details of NPA motifs and ar/R selectivity filters in aquaporins identified in Brassica napus.
| Gene ID | Aquaporin Name | NPA (LB) | NPA (LE) | H2 | H5 | LE1 | LE2 | NPA-NPA Distance |
|---|---|---|---|---|---|---|---|---|
| BnaA01g09720D | BnaANIP1-2a | NPA(114) | NPG(233) | W | V | A | R | 116 |
| BnaA03g43810D | BnaANIP1-2b | NPA(115) | NPG(234) | W | V | A | R | 116 |
| BnaA05g09450D | BnaANIP2-1a | NPA(104) | NPA(223) | V | V | A | R | 116 |
| BnaA05g09470D | BnaANIP2-1b | NPA(104) | NPA(223) | V | V | A | R | 116 |
| BnaA05g16540D | BnaANIP3-1a | NPA(67) | NPA(186) | W | I | A | R | 116 |
| BnaA05g16550D | BnaANIP3-1b | NPA(116) | NPA(211) | W | I | A | R | 92 |
| BnaA08g07040D | BnaANIP3-1c | NPA(75) | NPA(194) | W | I | A | R | 116 |
| BnaA04g08310D | BnaANIP4-1a | NPA(102) | NPA(214) | W | V | A | R | 109 |
| BnaA04g27980D | BnaANIP4-1b | NPA(54) | NPA(166) | W | V | A | R | 109 |
| BnaA02g22030D | BnaANIP5-1a | NPS(134) | NPV(245) | A | I | G | R | 108 |
| BnaA03g24370D | BnaANIP5-1b | NPS(134) | NPV(245) | A | I | A | R | 108 |
| BnaA07g16310D | BnaANIP5-1c | NPS(134) | NPV(245) | A | N | A | R | 108 |
| BnaA02g19440D | BnaANIP6-1a | NPA(139) | NPV(250) | A | I | A | R | 108 |
| BnaA02g36290D | BnaANIP6-1b | NPA(139) | NPV(250) | A | I | A | R | 108 |
| BnaA07g35330D | BnaANIP6-1c | NPA(139) | NPV(250) | A | I | A | R | 108 |
| BnaA05g31180D | BnaANIP7-1a | NPS(104) | NPA(216) | A | V | G | R | 109 |
| BnaA04g00710D | BnaAPIP1-1a | NPA(53) | NPA(174) | F | H | T | R | 118 |
| BnaA09g39170D | BnaAPIP1-1b | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaAnng23190D | BnaAPIP1-1c | NPA(34) | NPA(155) | F | H | T | R | 118 |
| BnaA03g21210D | BnaAPIP1-2a | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaA04g26560D | BnaAPIP1-2b | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaA05g05230D | BnaAPIP1-2c | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaA10g00360D | BnaAPIP1-3a | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaA03g27130D | BnaAPIP1-4a | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaA09g51960D | BnaAPIP1-4b | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaA03g45950D | BnaAPIP1-5a | NPA(115) | NPA(236) | F | H | T | R | 118 |
| BnaA09g33720D | BnaAPIP2-1a | NPA(107) | NPA(228) | F | H | T | R | 118 |
| BnaA03g17020D | BnaAPIP2-2a | NPA(105) | NPA(226) | F | H | T | R | 118 |
| BnaA03g17030D | BnaAPIP2-2b | NPA(105) | NPA(226) | F | H | T | R | 118 |
| BnaA05g07300D | BnaAPIP2-2c | NPA(105) | NPA(251) | F | H | T | R | 143 |
| BnaA02g06180D | BnaAPIP2-4a | NPA(80) | NPA(201) | F | H | T | R | 118 |
| BnaA03g08820D | BnaAPIP2-4b | NPA(82) | NPA(203) | F | H | T | R | 118 |
| BnaA10g13480D | BnaAPIP2-4c | NPA(82) | NPA(203) | F | H | T | R | 118 |
| BnaA07g16510D | BnaAPIP2-5a | NPA(106) | NPA(227) | F | H | T | R | 118 |
| BnaA09g34600D | BnaAPIP2-5b | NPA(106) | NPA(227) | F | H | T | R | 118 |
| BnaA03g18300D | BnaAPIP2-6a | NPA(105) | NPA(226) | F | H | T | R | 118 |
| BnaA08g10860D | BnaAPIP2-7a | NPA(101) | NPA(222) | F | H | T | R | 118 |
| BnaAnng11630D | BnaAPIP2-7b | NPA(101) | NPA(222) | F | H | T | R | 118 |
| BnaA07g17050D | BnaASIP2-1a | NPL(69) | NPA(180) | S | H | G | A | 108 |
| BnaA09g36250D | BnaASIP2-1b | NPV(69) | NPA(177) | S | H | G | A | 105 |
| BnaAnng22640D | BnaATIP1-1a | NPA(55) | NPA(169) | H | I | A | V | 111 |
| BnaAnng24130D | BnaATIP1-1b | NPA(55) | NPA(169) | H | I | A | V | 111 |
| BnaA02g28130D | BnaATIP1-2a | NPA(86) | NPA(200) | H | I | A | V | 111 |
| BnaA06g32840D | BnaATIP1-2b | NPA(86) | NPA(200) | H | I | A | V | 111 |
| BnaA09g51590D | BnaATIP1-3a | NPA(85) | NPA(199) | H | I | A | V | 111 |
| BnaA01g28120D | BnaATIP2-1a | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaA03g34110D | BnaATIP2-1b | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaA05g23460D | BnaATIP2-1c | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaA01g35340D | BnaATIP2-2a | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaA02g25440D | BnaATIP2-3a | NPA(43) | NPA(157) | H | I | G | R | 111 |
| BnaA06g40020D | BnaATIP2-3b | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaA02g16380D | BnaATIP3-1a | NPA(93) | NPA(207) | H | I | A | R | 111 |
| BnaA07g22790D | BnaATIP3-1b | NPA(93) | NPA(207) | H | I | A | R | 111 |
| BnaA07g30640D | BnaATIP3-1c | NPA(93) | NPA(207) | H | I | A | R | 111 |
| BnaA06g12030D | BnaATIP3-2a | NPA(93) | NPA(207) | H | M | A | R | 111 |
| BnaA09g44820D | BnaATIP3-2b | NPA(93) | NPA(207) | H | M | A | R | 111 |
| BnaA06g17390D | BnaATIP5-1a | NPA(87) | NPA(200) | N | V | G | C | 110 |
| BnaC01g11410D | BnaCNIP1-2a | NPA(114) | NPG(233) | W | V | A | R | 116 |
| BnaC07g35550D | BnaCNIP1-2b | NPA(115) | NPG(234) | W | V | A | R | 116 |
| BnaC04g10820D | BnaCNIP2-1a | NPA(104) | NPA(223) | V | V | A | R | 116 |
| BnaC04g10830D | BnaCNIP2-1b | NPA(104) | NPA(223) | V | V | A | R | 116 |
| BnaC05g29140D | BnaCNIP3-1a | NPA(99) | NPA(218) | W | I | A | R | 116 |
| BnaC05g29150D | BnaCNIP3-1b | NPA(116) | NPA(211) | W | I | A | R | 92 |
| BnaC08g07910D | BnaCNIP3-1c | NPA(102) | NPA(232) | W | I | A | R | 127 |
| BnaC04g30520D | BnaCNIP4-1a | NPA(102) | NPA(214) | W | V | A | R | 109 |
| BnaC04g34450D | BnaCNIP4-1b | NPA(102) | NPA(214) | W | V | A | R | 109 |
| BnaC06g42210D | BnaCNIP4-2a | NPA(107) | NPA(219) | W | V | A | R | 109 |
| BnaC02g29210D | BnaCNIP5-1a | NPS(134) | NPV(245) | A | I | G | R | 108 |
| BnaC03g28980D | BnaCNIP5-1b | NPS(134) | NPV(245) | A | I | A | R | 108 |
| BnaC06g40240D | BnaCNIP6-1a | NPA(139) | NPV(250) | A | I | A | R | 108 |
| BnaC05g45720D | BnaCNIP7-1a | NPS(104) | NPA(216) | A | V | G | R | 109 |
| BnaC08g31360D | BnaCPIP1-1a | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaC03g25510D | BnaCPIP1-2a | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaC04g04640D | BnaCPIP1-2b | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaC04g50590D | BnaCPIP1-2c | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaC05g00440D | BnaCPIP1-3a | NPA(152) | NPA(273) | F | H | T | R | 118 |
| BnaCnng08780D | BnaCPIP1-3b | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaC03g32130D | BnaCPIP1-4a | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaCnng02360D | BnaCPIP1-4b | NPA(114) | NPA(235) | F | H | T | R | 118 |
| BnaC07g38190D | BnaCPIP1-5a | NPA(115) | NPA(236) | F | H | T | R | 118 |
| BnaC06g14590D | BnaCPIP2-1a | NPA(107) | NPA(228) | F | H | T | R | 118 |
| BnaCnng31040D | BnaCPIP2-1b | NPA(107) | NPA(228) | F | H | T | R | 118 |
| BnaC04g08090D | BnaCPIP2-2a | NPA(105) | NPA(226) | F | H | T | R | 118 |
| BnaC04g08100D | BnaCPIP2-2b | NPA(105) | NPA(226) | F | H | T | R | 118 |
| BnaC03g11160D | BnaCPIP2-4a | NPA(82) | NPA(203) | F | H | T | R | 118 |
| BnaC09g53920D | BnaCPIP2-4b | NPA(82) | NPA(203) | F | H | T | R | 118 |
| BnaC06g15450D | BnaCPIP2-5a | NPA(106) | NPA(227) | F | H | T | R | 118 |
| BnaC08g25570D | BnaCPIP2-5b | NPA(106) | NPA(227) | F | H | T | R | 118 |
| BnaC03g21800D | BnaCPIP2-6a | NPA(105) | NPA(226) | F | H | T | R | 118 |
| BnaC01g03410D | BnaCPIP2-7a | NPA(101) | NPA(222) | F | H | T | R | 118 |
| BnaC03g65520D | BnaCPIP2-7b | NPA(101) | NPA(222) | F | H | T | R | 118 |
| BnaC07g45370D | BnaCPIP2-7c | NPA(74) | NPA(195) | F | H | T | R | 118 |
| BnaC03g54990D | BnaCSIP2-1a | NPL(69) | NPA(180) | S | H | G | A | 108 |
| BnaC04g24760D | BnaCSIP2-1b | NPL(69) | NPA(180) | S | H | G | A | 108 |
| BnaCnng20470D | BnaCSIP2-1c | NPL(69) | NPA(180) | S | H | G | A | 108 |
| BnaCnng24720D | BnaCTIP1-1a | NPA(85) | NPA(199) | H | I | A | V | 111 |
| BnaC02g36210D | BnaCTIP1-2a | NPA(86) | NPA(200) | H | I | A | V | 111 |
| BnaC07g23630D | BnaCTIP1-2b | NPA(86) | NPA(200) | H | I | A | V | 111 |
| BnaCnng01570D | BnaCTIP1-3a | NPA(85) | NPA(199) | H | I | A | V | 111 |
| BnaC01g44580D | BnaCTIP2-1a | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaC03g39560D | BnaCTIP2-1b | NPA(109) | NPA(223) | H | I | G | R | 111 |
| BnaC05g37160D | BnaCTIP2-1c | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaC06g05270D | BnaCTIP2-1d | NPA(82) | NPA(196) | H | I | G | R | 111 |
| BnaC01g41690D | BnaCTIP2-2a | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaC02g46870D | BnaCTIP2-3a | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaC07g20220D | BnaCTIP2-3b | NPA(83) | NPA(197) | H | I | G | R | 111 |
| BnaC06g23750D | BnaCTIP3-1a | NPA(93) | NPA(207) | H | I | A | R | 111 |
| BnaC06g34100D | BnaCTIP3-1b | NPA(93) | NPA(207) | H | I | A | R | 111 |
| BnaCnng50290D | BnaCTIP3-1c | NPA(93) | NPA(207) | H | I | A | R | 111 |
| BnaC05g13770D | BnaCTIP3-2a | NPA(93) | NPA(207) | H | M | A | R | 111 |
| BnaC08g37510D | BnaCTIP3-2b | NPA(93) | NPA(207) | H | M | A | R | 111 |
| BnaC04g38040D | BnaCTIP4-1a | NPA(79) | NPA(193) | A | I | A | R | 111 |
| BnaCnng15220D | BnaCTIP5-1a | NPA(87) | NPA(200) | N | V | G | C | 110 |
| BnaA01g33700D | BnaASIP1-1a | NPT(69)* | NPA(184)* | T | V | P | I | 112 |
| BnaA05g37440D | BnaASIP1-1b | NPT(69)* | NPA(184)* | T | V | P | I | 112 |
| BnaA10g16540D | BnaASIP1-2a | NPC(72)* | NPA(188)* | V | F | P | I | 113 |
| BnaCnng65250D | BnaCNIP4-1c | NP(107)* | — | P | — | — | — | — |
| BnaC01g40230D | BnaCSIP1-1a | NPT(71)* | NPA(186)* | T | V | P | I | 112 |
| BnaC05g47470D | BnaCSIP1-1b | NPT(69)* | NPA(184)* | T | V | P | I | 112 |
| BnaC09g54320D | BnaCSIP1-2a | NPC(72)* | NPA(188)* | V | F | P | I | 113 |
NPA motifs were identified using CDD search at NCBI. The NPA motifs with an asterisk (*) initially failed to be identified with CDD search and were then identified with manual evaluation of protein sequence alignments.