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. 2017 Jun 6;4:30. doi: 10.3389/fsurg.2017.00030

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Copyright © 2017 Ram, Brasch, Dunne, Davis, Tan and Itinteang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative 3,3-diaminobenzidine immunohistochemical-stained sections of moderately differentiated lip cancer samples showing cytoplasmic expression of (pro)renin receptor [(A), brown] within the tumor nests (TNs) and cells within the stroma. Angiotensin-converting enzyme [(B), brown] was expressed on the endothelium of the microvessels within the stroma but not the TNs. Angiotensin II receptor 1 [(C), brown] showed weak to moderate cytoplasmic and nuclear staining by cells within the TNs and cells within the stroma. Strong perinuclear and cytoplasmic staining for angiotensin II receptor 2 [(D), brown] was present in cells within the TNs and the stroma, and skeletal tissue. Nuclei were counterstained with hematoxylin [(A–D), blue]. Original magnification: 200×.