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Effects of aging and leonurine on capillary density and expressions of HIF and VEGF. (A) CD31 immunostaining of ischemic skeletal muscles harvested at post-surgical day 14 in each group. Scale bar = 50 μm. (B) Quantification of capillary density. (C,D) Effects of aging and leonurine on the expressions of HIF and VEGF in ischemic skeletal muscles assessed by Western blot. Data are mean ± SEM. ∗∗p < 0.01 vs. young group; ##p < 0.01 vs. old group. n = 5–8 for each group.
