Figure 3.

Determination of HSAF yield produced by L. enzymogenes in the presence or absence of P. aphanidermatum at 24, 48, 96 h. OH11, monoculture of the wild-type strain of L. enzymogenes. OH11-Pa, OH11 was co-cultured with P. aphanidermatum, as shown in Figure 1. HSAF production of OH11 was illustrated in Peak area/OD_600nm as means of three biological replicates, each containing two or three technical replicates. Peak area indicated the area of HSAF determined by HPLC method, while OD_600nm represents the growth status of tested strains at the time points used for the extraction of HSAF. Vertical bars indicated standard errors of three biological replicates. Significant difference in HSAF production between OH11 monoculture and co-cultured with P. aphanidermatum according to a t-test (^*p < 0.05, ^**p < 0.01).