Table 1.
Purification of Mo-CBP2.
| Purification stepsa | Total protein (mg)b | Yield (%)c |
|---|---|---|
| Crude extract | 205.41 ± 2.55 | 100 |
| Albumin | 112.45 ± 3.00 | 54.7 |
| Chitin chromatography | 47.57 ± 5.46 | 23.2 |
| CM-Sepharose chromatography (Mo-CBP2) | 0.32 ± 0.02 | 0.2 |
Results are the mean ± standard deviation of six similar runs. aPurification steps are described in Materials and Methods. bTotal amount of protein recovered from 1 g of defatted flour from M. oleifera seeds. cProtein recovery at each purification step (crude extract, 100%).