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. 2017 Jun 6;10:175. doi: 10.3389/fnmol.2017.00175

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Copyright © 2017 Wu, Ye, Zhou, Wang, Xie, Zhang, Gao, Zhang, Zhou, Zhuang, Liu, Hang and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-immunofluorescent staining shows that TUNEL-positive cells co-localized with the neurons marker (NeuN) in the overlapped images. It showed that the number of TUNEL-positive neurons was higher in SAH group as compared to that in Sham group. In LV-Panx1-EGFP group, the number of TUNEL-positive neurons was even much higher than SAH + LV-NC group. LV-ShRNA-Panx1 attenuates the level of TUNEL-positive neurons as compared with SAH + LV-Scramble-ShRNA group (TUNEL = red, NeuN = green, DAPI = blue, Scale bars 20 μm). Bars represent the mean ± SD (n = 6, each group; ^##P/^&&P < 0.01; ^∗∗∗P < 0.001; ^NSP > 0.05).