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. 2017 Jun 6;10:175. doi: 10.3389/fnmol.2017.00175

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Copyright © 2017 Wu, Ye, Zhou, Wang, Xie, Zhang, Gao, Zhang, Zhou, Zhuang, Liu, Hang and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The mRNA expressions of TLR2 and TLR4 were measured by Quantitative real-time PCR in each experimental group. The induction of SAH led to a significantly upward tendency in TLR2 and TLR4 mRNA expressions compared with Sham group. The mRNA expressions of TLR2 and TLR4 were significantly down-regulated with LV-ShRNA-Panx1 administration as compared with SAH + LV-Scramble-ShRNA group and markedly up-regulated with LV-Panx1-EGFP transfection as compared with SAH + LV-NC group. Bars represent the mean ± SD (n = 6, each group; ^∗P/^#P < 0.05; ^∗∗P/^##P < 0.01; ^∗∗∗P/^###P/^&&&P < 0.001; ^NSP > 0.05).