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. 2017 Jun 6;10:175. doi: 10.3389/fnmol.2017.00175

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Copyright © 2017 Wu, Ye, Zhou, Wang, Xie, Zhang, Gao, Zhang, Zhou, Zhuang, Liu, Hang and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression levels of apoptosis associated proteins were assessed by Western blot analysis.Quantitative analysis of representative autoradiogram of Bax, Bcl-2, and cleaved Caspase-3 shows that LV-ShRNA-Panx1 administration significantly suppressed the expression of apoptosis associated proteins as compared with SAH + LV-Scramble-ShRNA group. Likewise, in LV-Panx1-EGFP group, Bax and cleaved Caspase-3 protein levels were markedly higher than that in SAH + LV-NC group. β-actin was used as the loading control. Bars represent the mean ± SD (n = 6, each group; ^∗P/^#P/^&P < 0.05; ^∗∗P/^##P/^&&P < 0.01; ^∗∗∗P < 0.001; ^NSP > 0.05).