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. 2017 Jun 6;11:159. doi: 10.3389/fncel.2017.00159

FIGURE 1.

FIGURE 1

Developmental knockdown of na or unc79 disrupts evening behavior in adults. Normalized activity patterns of adult male Drosophila averaged over 4 days of light:dark (LD) entrainment conditions. Zeitgeber time indicated below each panel. White bars represent activity levels during light phase, while black bars indicate activity during dark phase. Arrows indicate normal timing of morning (black) and evening (gray) activity increases, and error bars represent standard error of the mean. (A–C) daGAL4 tubGAL80ts genotypes raised at 29°C and maintained at 29°C throughout the behavioral assay. For 29°C assays, flies were subject to 14 h light: 10 h dark conditions. (A) attP40 RNAi insertion control (n = 28). (B) UAS-na RNAi (attp2 locus; n = 33). (C) UAS-unc79 RNAi (attp40 locus; n = 42). (D–F) daGAL4 tubGAL80ts genotypes raised at 29°C and transferred to 19°C within 2 days of eclosion. For 19°C assays, flies were subject to 12 h light: 12 h dark conditions. (D) attP40 control (n = 46). (E) UAS-na RNAi (n = 27). (F) UAS-unc79 RNAi (n = 45).