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. 2017 Jun 6;11:159. doi: 10.3389/fncel.2017.00159

FIGURE 3.

FIGURE 3

Developmental requirement for Nlf-1 for adult rhythmic behavior. Normalized activity patterns of adult males averaged over 4 days of 12 h light: 12 h dark conditions (19°C behavioral assays) or 14 h light: 10 h dark conditions (29°C behavioral assays). Zeitgeber time indicated below each panel. White bars indicate light phase activity, black bars indicate dark phase activity, and error bars represent standard error of the mean. Where included, black arrows indicate morning activity and gray arrows evening activity. Each genotype is daGAL4 tubGAL80ts/+ with RNAi or control insertion as indicated. (A,B) Genotypes raised at 19°C and maintained at 19°C throughout the behavioral assay. (A) attP2 RNAi insertion control (n = 39). (B) UAS-Nlf-1 RNAi (attp2 locus; n = 37). (C,D) Genotypes raised and maintained at 29°C. (C) attP2 RNAi insertion control (n = 29). (D) UAS-Nlf-1 RNAi (attp2 locus; n = 27). (E,F) Genotypes raised at 19°C and transferred to 29°C on the 1st day of the behavioral assay. (E) attP2 RNAi insertion control (n = 34). (F) UAS-Nlf-1 RNAi (attp2 locus; n = 34). (G,H) Genotypes raised at 29°C and transferred to 19°C within 2 days of eclosion, at least 2 days prior to the start of the behavioral assay. (G) attP2 RNAi insertion control (n = 36). (H) UAS-Nlf-1 RNAi (attp2 locus; n = 28).