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. 2017 Jun 6;11:159. doi: 10.3389/fncel.2017.00159

Table 2.

Transgenic rescue of NA in adult circadian neurons promotes robust DD rhythms.

Genotype Temp. (Dev. – > Adult) Period ± SEM (Hours) Power ± SEM Rhythmic (%) n
nahar; tubGAL80ts; Clk8.0GAL4 19 – > 19 24.4 ± 0.6 2 ± 1 6 36
nahar; tubGAL80ts; Clk8.0GAL4/ UAS-na 19 – > 19 24.4 ± 0.6 6 ± 1 19&& 52
nahar;; Clk8.0GAL4/ UAS-na 19 – > 19 24.6 ± 0.1 61 ± 7 89∗∗ 28
nahar; tubGAL80ts; Clk8.0GAL4 29 – > 29 23.5 3 ± 2 8 13
nahar; tubGAL80ts; Clk8.0GAL4/ UAS-na 29 – > 29 24.1 ± 0.1 43 ± 6 83∗∗ 24
nahar;; Clk8.0GAL4/ UAS-na 29 – > 29 23.8 ± 0.0 42 ± 5 88∗∗ 33
nahar; tubGAL80ts; Clk8.0GAL4 19 – > 29 24.9 ± 0.5 8 ± 2 27 30
nahar; tubGAL80ts; Clk8.0GAL4/ UAS-na 19 – > 29 24.1 ± 0.1 62 ± 6 89∗∗ 37
nahar;; Clk8.0GAL4/ UAS-na 19 – > 29 24.0 ± 0.0 64 ± 7 88∗∗ 34
nahar; tubGAL80ts; Clk8.0GAL4/ UAS-na UAS-Nlf-1 RNAi 19 – > 29 24.8 ± 0.3 21 ± 6 46&& 13
nahar; tubGAL80ts; Clk8.0GAL4/ UAS-na UAS-Nlf1 19 – > 29 23.9 ± 0.2 26 ± 8&& 71 7
nahar; tubGAL80ts; Clk8.0GAL4 29 – > 19 21.5 1 ± 0 2 50
nahar; tubGAL80ts; Clk8.0GAL4/ UAS-na 29 – > 19 24.1 ± 0.2 10 ± 2 34∗∗&& 68
nahar;; Clk8.0GAL4/ UAS-na 29 – > 19 24.5 ± 0.2 33 ± 4 66∗∗ 62

Rhythmicity analyses from adult Drosophila males maintained in 5–6 days LD conditions followed by 7 days DD. Genotypes indicated in the left column. Temp. column indicates the temperature (°C) that crosses were raised at (Dev.) followed by the temperature of the adult behavioral assay. Power indicates chi-squared periodogram power minus significance. Significant differences from the corresponding mutant controls (nahar; tubGAL80ts; Clk8.0GAL4) and/or constitutive rescue (nahar; Clk8.0GAL4/UAS-na) are indicated by (∗∗p < 0.01) or & (&&p < 0.01), respectively. Significance was determined using Fisher’s exact test..