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. 2017 Apr 22;6(6):1–16. doi: 10.1093/gigascience/gix031

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© The Authors 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4: — Function approval of SmydA-2 genes through experimental evidence. (A)In vitro methyltransferase assay of histone H3 of SmydA-2 in locusts. Anti-pan methyl lysine antibody recognizes histone H3 in vitro methylated with SmydA-2. Anti-histone H3 serves as endogenous control for protein samples. The analyses were carried out in three replicates. **P < 0.01. (B) Expression evidence of SmydA-2 in the brain and cuticle of locusts via fluorescence in situ hybridization analysis. Green signals indicate the expression of SmydA-2/control, and blue signals indicate nuclear staining with Hoechst. (C) Relative gene expression of SmydA-2 in the different tissues. mRNA levels are quantified using the SYBR Green expression assays on a LightCycler 480 instrument. The qPCR data are shown as the mean ± SEM (n = 6). (D) Survival analysis of the locusts after SmydA-2 double-strand RNA injection. Data are analyzed through the Kaplan–Meier survival curve comparison of the dsSmydA-2 and dsGFP groups for three replicates.