Figure 1.

Generation of degalactosylated and galactosylated immunoglobulin gamma (IgG) isotypes derived from rituximab. (A) Schematic depiction of an IgG antibody with its two Fc N-glycans (up) and detailed glycan composition of a fully galactosylated IgG-Fc N-glycan. (B) Gel electrophoresis and coomassie staining to detect total protein showing heavy and light chains of antibodies (up) and immunoblots using galactose- (Erythrina Cristagalli lectin) or mannose- (Lens Culinaris agglutinin) specific lectins.