Figure 7. Stopping clock in brain and/or PG leads to arrhythmic adult emergence.

Pattern of emergence when all clocks (a), the brain clock (b) or the PG (c) clock, have been stopped. Schematic shown on the left represents the brain and PG clocks (large and small circle, respectively); red indicates which clock has been stopped; middle: pattern of eclosion in DD; right: autocorrelation analysis of record, with dominant periodicity and value of RI indicated. (e) Average RI (±s.e.m.) for genotypes shown in a–d and for controls; individual values are indicated when N<5 and average indicated by short horizontal line. Dashed line marks RI cutoff value of 0.1. Different letters indicate statistically different groups (P<0.05; one-way ANOVA, Tukey's post hoc multiple comparison analyses). Numbers in parenthesis indicate number of separate experiments. phm-gal80 effectively suppresses tim-gal4 expression in the PG (cf. Fig. 5d). (Strictly speaking, in (b) gene expression is driven in all clocks except the PG. However, since circadian rhythmicity of emergence depends only on the clocks in the brain and in the PG, such experiment is equivalent to driving gene expression only in the brain.)