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. 2017 May 30;8:15622. doi: 10.1038/ncomms15622

Figure 5. IsomiR-4534 guides Argonaute 1 binding to sense promoter-associated RNA.

Figure 5

(a) Binding of isomiR-4534 to HA-AGO1 and biotinylated S-paRNA (B S-paRNA) in RNA pull-down. S-paRNA, nonbiotinylated S-paRNA; Ctrl, control RNA. (b) IsomiR-4534 binding to HA-AGO1 in cells transfected with empty (EV) or S-paRNA expression vector determined by RIP. (c) IsomiR-4534 binding to chromatin-associated AGO1 determined by RNA-ChIP. (d) Predicted base pairing of isomiR-4534 with S-paRNA. Asterisks, edited bases in isomiR-4534; Red bases, nucleotides in putative canonical seed region. (e) Binding of AGO1 to S-paRNA with either wild-type (WT) or mutated isomiR-4534 binding sequence (BS) determined by RIP. CD44 mRNA, positive control. Top, red underlined bases indicate mutated bases in the BS construct. Data are mean±s.d. of three independent determinations. Student's t-test was used for P value assessment. *P<0.05.