Figure 3. Targeting individual genomic sites with more than one sgRNA.

Each of seven individual genomic sites (A, D-4(2), E-1, E-2, E-3, E-4 and E-5) was simultaneously targeted with two or three sgRNAs. Combined deletions of more than one genomics site (C-1/3 and D-1/2/3/4) were accomplished in two steps. Two sgRNAs targeting site C-1 were simultaneously injected into zygotes from mice carrying already a deletion in site C-3 (ref. ¹⁹). Two sgRNAs targeting site D-2 were simultaneously injected into mice carrying already a deletion in sites C-1/3/4. A, Stat5; C and D, Wap; E, Csn1s1. The red numbers refer to the sites being targeted in the respective experiments.