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. 2017 May 31;8:15464. doi: 10.1038/ncomms15464

Figure 7. Large deletions obtained with single sgRNAs.

Figure 7

(a) Diagram of single and multiple sgRNAs targeting specific sites. Targeted sites are shown in purple and sgRNAs are indicated as cyan arrows. (b) Comparison of deletion sizes generated by single sgRNAs and multiple sgRNAs injected into mouse zygotes (total number of founder mice, n=243; single sgRNA injection at a single site, n=122; multiple sgRNA injections at a single site, n=121). Single sgRNA injection at a single site, deletions obtained by targeting TF-binding site B, C-1, C-2/3, D-2, D-3, D-4(1) and F; multiple sgRNA injection at a single site, deletions obtained by targeting TF-binding site A, C-1/3, D-4(2), D-1/2/3/4, E-1, E-2, E-3, E-4 and E-5. The median deletion size obtained with single sgRNAs (9 bp) was smaller than that with multiple sgRNAs (84 bp). The deletion size generated with a single sgRNA was up to 600 bp. Median, middle bar inside the box; IQR, 50% of the data; whiskers, 1.5 times the IQR.