Figure 4. ZNHIT2 is a cofactor of R2TP/PFDL that targets the U5 snRNP.

(a) Diagram of the network of high-confidence interactions formed around ZNHIT2. Solid lines denote interactions with a FDR lower than 0.1 while dashed lines are interactions of relevance in this network with FDR scores higher than 0.1, but lower than 0.2 (Supplementary Data 1). Red arrows are target proteins identified in the ZNHIT2 purification. Coloured nodes are tagged subunits used in this experiment. White nodes are known protein complex subunits that were not detected. (b) CoImmunoprecipitation (CoIP) of FLAG-tagged subunits of the R2TP/PFD complex (RPAP3 and URI1) in HEK-293 cells following transfection with either non-specific (siCTRL) or ZNHIT2-targetting siRNAs (siZNHIT2). Various Western blots were made to detect endogenous or recombinant proteins, as indicated on the right side.