FIGURE 4.

Effect of BBr on inflammation mediators in the mPFC of DM rats. (A,B) BBr reduced the increased expression of p-IKK^{Y 199} and p- NF-κB^Ser536 in the mPFC of DM rats, n = 3 for western-blot. (C) The immunochemistry showed that the increased expression of GFAP in the mPFC of DM rats was largely inhibited by BBr, n = 4 for immunochemistry. (D–F) BBr decreased the inflammation cytokine IL-18 (D), but had no effect on IL-1β (E), and TNF-α (F) in the mPFC of DM rats, n = 8∼10 for ELISA. ^∗P < 0.05 vs. NOR; ^∗∗P < 0.01 vs. NOR; ^#P < 0.05 vs. DM. (G,H) BBr reduced the increased expression of p-ERK and p-JNK in the mPFC of DM rats, n = 3 for western-blot.