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. 2017 Jun 6;7:2864. doi: 10.1038/s41598-017-02856-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ruminal metabolomics pathway analysis by MetaboAnalyst 3.0 Software on the steers with the greatest average daily gain compare to the least ADG with similar average dry matter intake according to Bos taurus KEGG pathway database. UPLC-qTOF 90 metabolites identified to differ between ADG by t-test (P < 0.1). (a) Alpha-linolenic acid metabolism, (b) linoleic metabolism (c) taurine and hypotaurine metabolism d) phenylalanine, tyrosine and tryptophan (e) sphingolipids (f) phenylalanine (g) retinol (h) histidine (i) primary bile biosynthesis (j) glycerophospholipid (k) tyrosin (l) porphyrin and chlorophyll (m) steroid hormone biosynthesis (n) glutathione (o) Riboflavin (p) Gluconeogenesis. The darker the color and larger the size represent higher P-value from enrichment analysis and greater impact from the pathway topology analysis, respectively.