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. 2017 Jun 6;7:2839. doi: 10.1038/s41598-017-03194-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Synchrotron analysis of a leaflet initially treated with 6.7 mM AsV showing the distributions of As as compared to K and Ca. (B) Arsenic K-edge µ-XANES scans collected at various locations throughout the leaflet shown in 4 A (As), compared to AsV and AsIII reference spectra. The two dashed lines locate the energy positions of the edge maxima of As(III)2S3 and Na2HAs(V)O4. The location of the spots are detailed in Supplementary Information Fig. 1. (C) Synchrotron X-ray fluorescence images of As, S and K in pinna tissue cross-section (2 mm from apex) from 6.7 mM treatment showing elevated levels of As adjacent to mid-rib vein and secondary veins with preferential accumulation on abaxial (lower) surface of the pinnae.