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. 2017 Jun 6;7:2842. doi: 10.1038/s41598-017-03142-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of temperature on PDE activity. The activity of Sb-PDE was measured using the standard assay mixture with BpNpp as the substrate at 25–70 °C. The final concentration of purified Sb-PDE in the assay mixture was 0.07 µg/ml. The data are means ± standard deviations from three independent experiments.