Figure 9.

Co-localization of GFAP/brain-derived neurotrophic factor (BDNF) after carotid arteries occlusion. Results are shown for Sham group (A–C), mice after carotid arteries occlusion (D–F), mice treated with co-ultraPEALut (G–I). Brain sections were double stained with antibodies against GFAP [(A,D,G), green], and BDNF [(B,E,H), red]. Hippocampal area revealed increased astrogliosis (D) in VaD group. GFAP immunoreactivity was reduced in co-ultraPEALut-treated mice (G). Yellow spots indicate co-localizations and revealed a high co-localization between GFAP/BDNF double staining (C,F,I). The pictures are demonstrative of at least three experiments executed on distinctive experimental days. Images are representative of all the animals in every group. All pictures were digitalized at a resolution of 8 bits into an array of 2,048 × 2,048 pixels.