Fig. 6.
Effect of ON012380 on the in vivo growth of T315I cells. (A) Nude mice (10 mice per group) were injected i.v. through the tail vein with 1 × 106 32D/BCR-ABLT315I cells. Treatment with daily i.p. injections of 100 mg/kg ON012380, 100 mg/kg imatinib, or an equal volume of saline was initiated 24 h later. Blood smears from each mouse were performed on days 7 and 14, and the number of T315I-expressing cells per 10 fields was determined. The data were plotted as the average number of 32D/BCR-ABLT315I cells per 10 fields ± SEM (n = 10). (B) The total body weight of individual mice in the three groups was determined daily, and the average body weights were plotted as the percent of starting body weight. (C) CD-1 mice were injected i.v. (tail vein injection) with saline or ON012380 (200 mg/kg) dissolved in saline. Bone marrow cells were extracted from the mice after 24 h, and 2 × 105 cells were plated on methycellulose containing appropriate cytokines for lineage-specific colony formation. Colonies were counted after 5–14 days of incubation. BFU-E, erythroid burst-forming unit; CFU-G, granulocyte colony-forming unit; CFU-M, macrophage colony-forming unit; CFU-GM, granulocyte/macrophage colony-forming unit; pre-B, pre-B lymphocyte.