Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 6;7:2865. doi: 10.1038/s41598-017-02930-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Site-directed mutagenesis into the BlaI/MecI binding sequence. (a) Sequence alignment of the blaZ/sodp and its constitutively induced blaZ/sodp(Con) promoter region. Asterisks indicate the sequence exchanged by site-directed mutagenesis. Bold face indicates the BlaI/MecI binding motif (TACA/TGTA) within the larger palindromes, the R1 dyad and Z dyad are indicated by arrows. Up arrows with the tip to right indicates the transcription initiation site. Underlined sequences indicate −35 and −10 elements. Double underline indicates the RBS sequence. Initiation codons are shown in uppercase. (b) The fluorescing colonies were photographed under UV excitation. S. aureus RN4220, MW2 and N315 containing either pFK52, or pFK54 were grown on TSB agar plate containing chloramphenicol. (c) The comparison of fluorescence intensity among S. aureus strain RN4220, MW2, and N315 containing either pFK52, or pFK54. The fluorescent intensities at 513 nm were measured with a microplate reader, λ_ex = 490 nm. The data represent mean values ± standard deviation. (d) The comparison of GFP_sa expression efficiency among S. aureus strain RN4220, MW2, and N315 containing either pFK52, or pFK54. SDS-PAGE and Western blot analysis show the relative quantities of GFP_sa in the whole cell lysates. The arrow indicates the position of GFP_sa in gel. The Western blot gel was cropped and the full-length image is included in Supplemental Fig. 5(b).