Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 6;7:2865. doi: 10.1038/s41598-017-02930-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Codon usage optimization of the amCyan and mCherry genes. (a) The fluorescing colonies were photographed under UV excitation. S. aureus strain MW2 containing pKAT (control), pFK62 (AmCyan), pFK64 (AmCyan(S.a)), pFK63 (mCherry), and pFK65 (mCherry(S.a)) were grown on TSB agar plates containing chloramphenicol. (b) SDS-PAGE analysis showed the relative quantities of AmCyan and mCherry in the whole cell lysates. Arrows indicate the position of AmCyan and mCherry in gel. (c) Western blot analysis of AmCyan or mCherry in the whole cell lysates. The Western blot gels were cropped and the full-length image are included in Supplemental Fig. 5(d). The comparison of fluorescent intensities of pKAT (control), pFK62 (AmCyan), and pFK64 (AmCyan(S.a)) in S. aureus MW2. The fluorescent intensities at 489 nm were measured with a microplate reader, λ_ex = 458 nm. The data represent mean values ± standard deviation. (e) The comparison of fluorescent intensities of pKAT (control), pFK63 (mCherry), and pFK65 (mChaerry(S.a)) in S. aureus MW2. The fluorescent intensities at 610 nm were measured with a microplate reader, λ_ex = 586 nm. The data represent mean values ± standard deviation.