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. 2017 Jun 6;7:2865. doi: 10.1038/s41598-017-02930-7

Figure 5.

Figure 5

Confocal laser microscopic analysis in co-culture systems. (a) Co-culture of three clinically isolated S. aureus strains. S. aureus strain N315 containing pFK60 (Cerulean), strain TY34 containing pFK56 (Citrine), and strain MW2 containing pFK65 (mCherry(S.a)) were co-cultured in BHI broth. Panels show (1) Cerulean (N315), (2) Citrine (TY34), (3) mCherry(S.a) (MW2), (4) overlay of Cerulean, Citrine, and mCherry images, (5) DIC, (6) overlay of Cerulean, Citrine, mCherry, and DIC images. (b) Co-culture of S. aureus with E. coli. S. aureus N315 containing pFK65 (mCherry(S.a)) and E. coli DH5α containing pFK55 (EGFP) were co-cultured in BHI broth. Panels show (1) DAPI, (2) EGFP, (3) mCherry(S.a), (4) DIC, (5) overlay of DAPI and DIC images, and (6) overlay of EGFP, mCherry(S.a), and DIC images.