Table 1.
Bacterial strains and plasmids used in this study.
| Bacterial strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | F−, φ80dlacZΔM15, Δ(lacZYA−argF)U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ−, thi-1, gyrA96, relA1 | TaKaRa |
| S. aureus | ||
| RN4220 | NCTC8325-4, r- m+ | 20 |
| N315 | hospital-aquired MRSA | 25 |
| MW2 | community-aquired MRSA | 26 |
| TY34 | clinical isolate MRSA from patient with impetigo | 35 |
| Plasmids | ||
| pMK4 | Shuttle vector between E. coli and S. aureus, Amr in E. coli, Cmr in S. aureus | 46 |
| pS1GFP | pMK4 containing GFPmut3b gene fused to the blaZp promoter | 19 |
| pND50 | Shuttle vector between E. coli and S. aureus, Cmr | 44 |
| pKAT | pND50 derivative containing the lacZ(α) gene from pUC19, Cmr | 43 |
| pAmCyan | Plasmid encoding AmCyan gene, Ampr | TaKaRa Clontech |
| pmCherry | Plasmid encoding mCherry gene, Ampr | TaKaRa Clontech |
| pFK51 | pMK4 containing GFPmut3b gene fused to the blaZ/sodp promoter | This study |
| pFK52 | pMK4 containing GFPsa gene fused to the blaZ/sodp promoter | This study |
| pFK53 | pMK4 containing GFPmut3b gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK54 | pMK4 containing GFPsa gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK55 | pMK4 containing EGFP gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK56 | pMK4 containing Citrine gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK57 | pMK4 containing EBFP gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK58 | pMK4 containing BFP(P4-3E) gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK59 | pMK4 containing BFP(1EMF) gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK60 | pMK4 containing Cerulean gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK61 | pMK4 containing mEmerald gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK62 | pKAT containing AmCyan gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK63 | pKAT containing mCherry gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK64 | pKAT containing codon-optimized AmCyan(S.a) gene fused to the blaZ/sodp(Con) promoter | This study |
| pFK65 | pKAT containing codon-optimized mCherry(S.a) gene fused to the blaZ/sodp(Con) promoter | This study |