Table 2.
Oligonucleotide primers.
| Name | primer sequence (5' to 3') | Purpose |
|---|---|---|
| blaZPR | AAATAATCATCCTCCTATTACAGTTGTAA | replacement of RBS sequence |
| GFP-F | ATGAGTAAAGGAGAAGAACTTTTCAC | |
| blaZPmut-F | TTTGTAAAAATATACACTTGAATAGGAGGATGAT | destruction of BlaI motif |
| blaZPmut-R | TCAATAATATTACCATTATGATATTGATG | |
| F64LS65T-R | TTGAACACCATATGTTAAAGTAGTAACAAG | construction of Emerald and EGFP |
| F64LY66H-R | TTGAACACCATGAGATAAAGTAGTAACAAG | construction of BFP(P4-3E) and BFP(1EMF) |
| F64LS65TY66H-R | TTGAACACCATGTGTTAATGTAGTAACAAG | construction of EBFP |
| F64LS65TY66W-R | TTGAACACCCCATGTTAAAGTAGTAACAAGTGTTG | construction of Cerulean |
| S65GV68LQ69M-R | CATTAAACCATAACCAAATGTAGTAACAAG | construction of Citrine |
| S72-F | TGTTTTTCAAGATATCCAGATCATATG | construction of EGFP and BFP |
| S72A-F | TGTTTTGCAAGATATCCAGATCATATG | construction of Cerulean, Emerald, and Citrine |
| F99S-F | TTCAAAGATGACGGTAACTACAAGAC | construction of GFPsa |
| F99S-R | AGATATAGTTCTTTCCTGTACATAACC | construction of GFPsa |
| Y145F-R | GATATATACATTATGTGAGTTAAAGTTATATTC | construction of EBFP, P4-3E(BFP) |
| Y145AN146IH148D-R | AATATATACATTGTCTGAGATAGCGTTATATTCCAA | construction of Cerulean |
| N149KM153T-R | CTTTTGTTTGTCTGCTGTAATATATACTTTATGTGAG | construction of Emerald |
| I152-R | AATATATACATTATGTGAATTATAGTTATATTCC | construction of GFPsa and BFP(1EMF) |
| M153-F | ATGGCAGACAAACAAAAGAATGGAATC | construction of EBFP, P4-3E(BFP) |
| M153TV163A-F | ACAGCAGACAAACAAAAGAATGGAATCAAAGCTAAC | construction of GFPsa and Cerulean |
| V163A-F | ATGGCAGACAAACAAAAGAATGGAATCAAAGCTAAC | construction of 1EMF(BFP) |
| I167T-F | AATGGAATTAAAGTTAACTTCAAAACAAGACAC | construction of Emerald |
| T203Y-F | TATCAATCTGCATTATCAAAAGATCCAAAC | construction of Citrine |
| T203Y-R | TGACAAATAATGATTGTCTGGTAAAAGAAC | construction of Citrine |
| A206K-F | ACACAATCTAAATTATCAAAAGATCCAAACG | monomerization |
| gapRF | AGAGAGGATCCTTAAATAGTTAGTTG | amplification of gapR promoter |
| gapRGFPR | GAAAAGTTCTTCTCCTTTACTCATTACTACCTCCTCCTTATATTTATA | amplification of gapR promoter fused to GFP |
| gapRGFPF | TATAAATATAAGGAGGAGGTAGTAATGAGTAAAGGAGAAGAACTTTTC | amplification of gfp gene fused to gapR promoter |
| GFPRB | GTCTAGATCTTTATTTGTATAGTTCATC | amplification of gfp gene |
| blaZPF | ACAAAAGCTTACTATGCTCATTATTAA | amplification of blaZ gene promoter |
| blaZPR-Cyan | AACTTGTTTGAAAGAGCCATAAATAATCATCCTCCTATTA | amplification of blaZ gene promoter fused to AmCyan |
| blaZP-CyanF | TAATAGGAGGATGATTATTTATGGCTCTTTCAAACAAGTT | amplification of amCyan gene fused to blaZ promoter |
| blaZPR-mCherry | TCCTCGCCCTTGCTCACCATAAATAATCATCCTTCCTATTA | amplification of blaZ gene promoter fused to mCherry |
| blaZP-mCherryF | TAATAGGAGGATGATTATTTATGGTGAGCAAGGGCGAGGA | amplification of mCherry gene fused to blaZ promoter |
| pUC-RH | AATGGAAGCTTCCGGCGCTCAGTTGG | amplification of amCyan and mCherry |
| Cyan1F | CATATGAAGGTACACAAACATCAACTTTTAAAGTTACAATGGCAAACGGTGGTCCACTTGCATTCTCATT | codon optimization for amCyan |
| Cyan1R | GTTTACCACTACCTTCACCTTTAACTGTAAAATAATGACCGTTAACACAACCATCCATATGATATGT | |
| Cyan2F | ATGCCAGATTATTTTAAACAAGCATTTCCTGATGGTATGTCATATGAACGTACTTTTACA | codon optimization for amCyan |
| Cyan2R | ACTTGTAGGATATGCAGTAAAACAACGATTACCATACATAAAAACTGTTGATAGAATAC | |
| Cyan3F | GAACATAAATCAACATTTCATGGAGTTAACTTTCC | codon optimization for amCyan |
| Cyan3R | AAAACAGTTACCTTTAAGACTTATTTCCCAACTTGC | |
| Cyan4F | CAAGGAGGTGGTAATTATAGATGTCAATTTCATACTTCTTATAAGACA | codon optimization for amCyan |
| Cyan4R | TAACATTAAAAATGCTGTAACATCACCCTTCAATATTCCATCACAAACAGTC | |
| Cyan5F | AAGGTGGTAATAGTGTTCAATTAACAGAACATGCTGTTGCACATATAACATCTGTTGTTCC | codon optimization for amCyan |
| Cyan5R | TATCTAAATCTGTTCTTGCAATACGATGTTCAACTGCATGGTTTGGTGGCATTGTAACTGGTTT | |
| Cherry1F | AGTTAATGGTCATGAATTCGAAATCGAGGGCGAGGGCGAGGGTCGTCCATATGAGGGCACACAAACAGC | codon optimization for mCherry |
| Cherry1R | GAACCTTCCATATGAACTTTAAAACGCATGAACTCCTTGATGATTGCCATGTTATCCTCCTCACCCTTAC | |
| Cherry2F | TATGGTTCAAAAGCATATGTTAAGCATCCAGCAGACATCCCAGACTATTTGAAGTTGTCATTCCCAGAGG | codon optimization for mCherry |
| Cherry2R | CATAAATTGAGGTGATAAGATGTCCCATGCGAATGGCAATGGACCACCCTTTGTCACCTTCAACTTTGC | |
| Cherry3F | TATTTATAAAGTTAAGTTGCGTGGTACAAACTTCCCATCAGACGGCCCAGTAATGCAGAAGAAGACAATG | codon optimization for mCherry |
| Cherry3R | AATTCACCATCTTGCAATGATGAGTCTTGTGTCACTGTAACCACACCGCCGTCCTCGAAGTTCATCACAC |