Table 1. Percentage of pituitary cells with single or multiple GnRH-R transcripts.
| PRL | GH | SL | FSH | LH | TSH | MSH | ACTH | ||
|---|---|---|---|---|---|---|---|---|---|
| R1 | IM | 20.8 ± 4.1 | 11.4 ± 7.0 | 20.5 ± 7.2 | 16.1 ± 8.4** | 20.0 ± 5.0 | 0.0 | 23.2 ± 6.2 | 0.0 |
| M | 18.7 ± 6.4 | 23.8 ± 7.5 | 9.0 ± 5.6 | 38.1 ± 10.5 | 12.5 ± 4.0 | 46.0 ± 11.7 | 35.0 ± 4.7 | 31.9 ± 8.1 | |
| R2 | IM | 13.9 ± 4.5 | 9.1 ± 3.7 | 6.2 ± 3.8 | 2.2 ± 2.2 | 7.5 ± 5.0 | 0.0 | 2.9 ± 2.9 | 0.0 |
| M | 6.7 ± 4.1 | 18.1 ± 7.4 | 0.0 | 15.7 ± 7.0 | 15.0 ± 4.7 | 0.0 | 2.5 ± 2.5 | 9.0 ± 5.9 | |
| R3 | IM | 2.2 ± 2.2 | 6.7 ± 6.7 | 8.6 ± 5.7 | 13.6 ± 5.4 | 12.5 ± 4.0 | 0.0 | 10.7 ± 5.3 | 0.0 |
| M | 3.3 ± 3.3 | 6.7 ± 4.1 | 5.0 ± 5.0 | 9.0 ± 3.7 | 12.5 ± 0.0 | 3.3 ± 3.3 | 10.0 ± 4.7 | 11.4 ± 5.3 | |
| R1+R2 | IM | 11.9 ± 4.0 | 12.9 ± 6.2 | 11.4 ± 11.4** | 24.7 ± 9.5* | 12.5 ± 4.0 | 0.0 | 22.5 ± 6.1 | 0.0 |
| M | 24.0 ± 8.4 | 2.9 ± 2.9 | 42.0 ± 10.1 | 6.2 ± 3.8 | 2.5 ± 2.5 | 0.0 | 12.5 ± 5.6 | 11.9 ± 8.4 | |
| R1+R3 | IM | 8.9 ± 4.2 | 8.6 ± 3.5 | 6.2 ± 3.8 | 2.5 ± 2.5 | 10.0 ± 2.5 | 0.0 | 10.0 ± 2.5 | 0.0 |
| M | 3.3 ± 3.3 | 3.3 ± 3.3 | 5.0 ± 5.0 | 0.0 | 5.0 ± 5.0 | 19.3 ± 9.0 | 12.5 ± 5.6 | 0.0 | |
| R2+R3 | IM | 2.2 ± 2.2 | 6.2 ± 3.8 | 2.9 ± 2.9 | 2.2 ± 2.2 | 10.0 ± 7.3 | 0.0 | 2.5 ± 2.5 | 0.0 |
| M | 3.3 ± 3.3 | 0.0 | 5.0 ± 5.0 | 0.0 | 15.0 ± 9.2 | 0.0 | 0.0 | 2.9 ± 2.9 | |
| R1+R2+R3 | IM | 16.9 ± 7.4 | 9.1 ± 3.7 | 6.2 ± 3.8 | 25.3 ± 4.7** | 7.5 ± 5.0 | 0.0 | 12.9 ± 4.0 | 0.0 |
| M | 24.7 ± 12.8 | 3.3 ± 3.3 | 22.0 ± 10.2 | 3.3 ± 3.3 | 10.0 ± 2.5 | 19.3 ± 6.4 | 0.0 | 0.0 | |
| R-positive | IM | 77.0 ± 6.5 | 63.8 ± 7.5 | 61.9 ± 5.4 | 88.9 ± 6.1 | 80.0 ± 3.1 | 0.0 | 84.6 ± 4.6 | 0.0 |
| M | 84.0 ± 11.7 | 58.1 ± 11.9 | 88.0 ± 8.0 | 72.4 ± 6.3 | 72.5 ± 7.3 | 88.0 ± 8.0 | 72.5 ± 7.3 | 67.1 ± 7.7 | |
| R-negative
|
IM | 23.0 ± 6.5 | 36.2 ± 7.5 | 38.1 ± 5.4 | 11.1 ± 6.1* | 20.0 ± 3.1 | 0.0 | 15.4 ± 4.6 | 0.0 |
| M | 16.0 ± 11.7 | 41.9 ± 11.9 | 12.0 ± 8.0 | 27.6 ± 6.3 | 27.5 ± 7.3 | 12.0 ± 8.0 | 27.5 ± 7.3 | 32.9 ± 7.7 |
R-positive and R-negative represent the total percentage of cells with and without GnRH-Rs, respectively. Statistical analysis on an animal basis (n = 5 per age group) show significant differences in the percentage of SL and FSH cells expressing GnRH-Rs in immature and mature males (bolded values). *, P<0.05; **, P<0.01, Fisher's probable least-squares difference test.