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. 2017 Jun 6;7:2867. doi: 10.1038/s41598-017-03032-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative in vivo images of astrocytes and quantitative analysis of astrocyte morphology features in the Ctrl group. Under normal physiological conditions, astrocytes typically have a characteristic bushy appearance consisting of thin process (A, A1). No obvious astrocytes vacuolization and morphological changes (B–F, B1–F1) were observed over a one-week period. No significant changes in mean fluorescence intensity (G), astrocyte number (H), astrocyte size (I), soma size (J) and soma-to-astrocyte ratio (K) occurred during a one-week period. (n = 6; one-way ANOVA or Kruskal-Wallis test, p > 0.05). The arrows in the lower magnification images indicate the astrocytes displayed in the higher magnification images.