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. 2017 Jan 25;3:7. doi: 10.1038/s41526-016-0004-6

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© The Author(s) 2017

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Fig. 2 — Srontium (SR) acts as a negative regulator of adipogenesis (AD) and a positive regulator of osteogenesis (OB): a Oil Red staining in AD conditions. b Alkaline phosphatase staining in OB conditions, c Adipogenesis markers were measured by RT-quantitative PCR during the culture [peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding proteins α (C/EBPα), fatty acid-binding protein (FABP4) and adiponectin], and d osteogenesis markers [alkaline phosphatase (ALP), osterix (OSX), bone sialoprotein (BSP) and osteopontin (OPN)], in AD or OB media, with or without 5 mM strontium. Day 8 of control static condition corresponds to the D8 CTL data. Error bars represent S.E.M. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to media without strontium or compared with conditions indicated by the horizontal bars (N = 3 independent experiments)