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. 2017 Jan 25;3:7. doi: 10.1038/s41526-016-0004-6

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© The Author(s) 2017

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Fig. 3 — Strontium (SR) regulates RhoA and Rac-1 activities in commited adipocytes and osteoblasts: C3H10T1/2 cells were cultured 2, 6 and 8 days in adipogenesis (AD) or osteogenesis (OB) media and simulated-microgravity, with or without 5 mM strontium. Cells were then harvested for G-LISA assays of RhoA (a) and Rac1 (b). Day 8 of control static condition corresponds to the D8 CTL data. Positive controls for RhoA and Rac1 activities are provided in the assay kit, activities of RhoGTpases data were normalized by this positive control. Error bars represent S.E.M. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to media without strontium, or compared to conditions indicated by the horizontal bars (N = 4independant experiments)