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. 2017 Jun 6;7:2816. doi: 10.1038/s41598-017-02559-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of recombinant CmLOX18 (a) Western blot analysis. Total proteins (20 μg) were isolated from yeast cells expressing CmLXO18 which was harvested at 24 hours induction and separated on 12% SDS/PAGE gel, then transferred to a PVD membrane and LOX detected with anti-His antibody. (b) SDS-PAGE analysis of the recombinant CmLOX18. The proteins were stained with Coomassie brilliant blue R-250. M: Positions of marker protein. 1: SDS-PAGE of the purified CmLOX18 extracted from pYES2/lox transformed yeast cultures (20 μg). The expressed CmLOX18 was indicated by red arrows.