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. 2017 Jun 6;7:2816. doi: 10.1038/s41598-017-02559-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of positional specificity of the recombinant CmLOX18. (a) The retention time of the isomers produced by soybean LOX1 compared with authentic standards of 13-HPOD and 9-HPOD. (b) SP-HPLC analysis of the reaction mixture catalysed by recombinant CmLOX18. Boxes: chiral-phase HPLC showing the enantiomer composition of 13-HPOD.