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. 2017 Jun 6;7:2816. doi: 10.1038/s41598-017-02559-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular analysis of T0 transegenic tomato liners expressing CmLOX18. (a) Southern blot of wild-type control and four T0 transgenic tomato lines expression CmLOX18. Genomic DNA was prepared from young leaf material from wild-type control and transgenic plants: 1, 2, and 3 and V (35S-overexpression CmLOX18 construct). The genomic DNA (10 μg/lane) was digested with HindШ and separated in a 0.8% (w/v) agarose gel. Blotted DNA was hybridized to a probe prepared from the bar gene. (b) Detection of CmLOX18 cDNA by PCR analysis. Genomic DNA was extracted from transgenic lines. (c) Western blot. Proteins were extracted and Western blot was carried according to standard procedures using anti-e GFP Mouse Monoclonal antibody. (d) Images of wild-type control and twoT0 transgenic tomato leaves.