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Kinetic parameters of purified recombinant CmLOX18.
| Substrate | Km (μM) | Vmax (μmol s−1) | Kcat (s−1) | Kcat/Km (s−1 μM−1) |
|---|---|---|---|---|
| Linoleic acid | 126.9 ± 18.6 | 32.87 ± 3.1 | 4383 ± 295 | 34.54 ± 2.7 |
| Linolenic acid | 40.72 ± 2.65 | 8.19 ± 0.8 | 1098 ± 78 | 26.96 ± 2.2 |
Kinetic parameters were calculated using a Michaelis-Menten plot analysis program. Values represent the mean ± SD of 3 independent replicates. Kcat is defined to equal Vma/Et. Et is total enzyme concentration is molar.
