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. 2017 Jun 6;7:2830. doi: 10.1038/s41598-017-02116-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetic in BCG-induced mycobacterial growth inhibition. Groups of mice were immunised with a single dose BCG or given Tris buffer (Placebo) three times s.c. with 2-week intervals. In different immunisation experiments, splenocytes were isolated 1 week, 5 weeks or 29 weeks after BCG immunisation and used in MGIA. Delta log₁₀CFU values represent the BCG-induced mycobacterial growth inhibition and were calculated by subtracting the individual log₁₀CFU values in the immunised BCG groups from the mean of the respective placebo group. Solid lines represent the mean delta log₁₀CFU of 4, 8 and 4 mice, for 1 week, 5 weeks or 29 weeks, respectively, measured in duplicates. t-test between the BCG group and the respective placebo group. *p < 0.05; ****p < 0.0001.