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. 2017 Jun 6;7:2866. doi: 10.1038/s41598-017-03128-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a–f) PGMA and (g–l) PGMA-PEI nanoparticles in the ED10 conceptus. (a) Representative tissue section of a conceptus from a PGMA treated dam. (b) Outline of section denoting tissue regions. (c–e) Higher magnification images of the areas shown in the rectangles in (a). (d,f) High magnification images of panels (c) and (e) respectively. PGMA nanoparticles (indicated by arrowheads) were observed in the ectoplacental cone as well as in the primary decidual zone. (g) Representative tissue section of a conceptus from a PGMA-PEI treated dam. (h) Outline of section denoting tissue regions. (I,k) Higher magnification images of the areas shown in the rectangles in (g). (j,l) High magnification images of panels (i) and (k) respectively. PGMA-PEI nanoparticles (indicated by arrowheads) were observed in the decidua, specifically in the venous sinusoids and tissue close to the ectoplacental cone as well as in the primary decidual zone. Scale bars: (a,g) 1000 µm; (c,e,i,k) 200 µm; (d,f,j,l) 50 µm. De: decidua; ch: chorion; epc: ectoplacental cone; pdz: primary decidual zone. Images are representative of n = 4 per group.