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. 2017 Jun 6;7:2866. doi: 10.1038/s41598-017-03128-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a–d) PGMA and (e–h) PGMA-PEI nanoparticles in the placenta at ED20. (a) Representative tissue section of a placenta from a PGMA treated dam with key tissue regions indicated (n = 4 per group). (b–d) Higher magnification images of the areas indicated by the rectangles in (a). PGMA nanoparticles (indicated by arrowheads) were observed in the (b) chorionic plate, in the tissue adjacent to the (c) junctional zone and in the (d) labyrinth zone. (e) Representative tissue section of a placenta from a PGMA-PEI treated dam with key tissue regions indicated (n = 4 per group). (f–h) Higher magnification images of the areas indicated by the rectangles in (e). PGMA-PEI nanoparticles (indicated by arrowheads) were observed in the (f) chorionic plate, in the tissue adjacent to the (g) junctional zone and in the (h) labyrinth zone. Scale bars: (a,e) 1000 µm; (b–d,f–h) 200 µm. JZ: junctional zone; LZ: labyrinth zone; cp: chorionic plate.